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Lysobacter arvi sp. nov. Isolated from Farmland Soil

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Lysobacter arvi sp. nov. Isolated from Farmland Soil
 
Creator Choksket, Stanzin
Rana, Rekha
Patil, Prabhu B
Korpole, Suresh
 
Subject QR Microbiology
 
Description A bacterial strain designated as UC was isolated from farmland soil. Strain UCT formed a pale yellow colony on nutrient agar. Cell morphology revealed it as the rod-shaped bacterium that stained Gram-negative. The 16S rRNA gene sequence analysis identified strain UCT as a member of the genus Lysobacter that showed high identity with L. soli DCY21T (99.5%), L. panacisoli CJ29T (98.7%), and L. tabacisoli C8-1T (97.9%). It formed a distinct cluster with these strains in the neighbor-joining phylogenetic tree. A similar tree topology was observed in TYGS-based phylogenomic analysis. However, genome sequence analyses of strain UCT showed 87.7% average nucleotide identity and 34.7% digital DNA-DNA hybridization similarity with the phylogenetically closest species, L. soli DCY21T. The similarity was much less with other closely related strains of the genus Lysobacter. The G + C content of strain UCT was 68.1%. Major cellular fatty acids observed were C14:0 iso (13.4%), C15:0 iso (13.6%), and C15:0 anteiso (14.8%). Quinone Q-8 was the major respiratory ubiquinone. Predominant polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. Production of xanthomonadin pigment was observed. Based on phenotypic differences and phylogenomic analysis, strain UCT represents a novel species of the genus Lysobacter, for which the name Lysobacter arvi is proposed. The type strain of the novel species is UCT (= KCTC 92613T = JCM 23757T = MTCC 12824T).
 
Publisher Springer Science
 
Date 2023-10-25
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s00284-023-03486-8
http://crdd.osdd.net/open/3170/
 
Identifier Choksket, Stanzin and Rana, Rekha and Patil, Prabhu B and Korpole, Suresh (2023) Lysobacter arvi sp. nov. Isolated from Farmland Soil. Current microbiology, 80 (12). ISSN 1432-0991