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Therapeutic potentials of characterized isolate from column chromatography by GC-MS and molecular docking of principle active of Prunus domestica L.

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Title Therapeutic potentials of characterized isolate from column chromatography by GC-MS and molecular docking of principle active of Prunus domestica L.
 
Creator Shukla, Rishi Kumar
Shukla, Abha
Budhlakoti, Pankaj
 
Subject Prunus domestica L
Column chromatography
GC-MS
Antioxidant activity
Antibacterial activity
Molecular docking
 
Description 692-700
Chromatographic techniques aid in isolating and separating phytoconstituents from natural products, enabling the discovery of innovative compounds of pharmacological and physiological importance. The present study is focused on isolation and identification of the compounds in column chromatography-derived fraction using GC-MS and its biological activities. The ethyl acetate extract of the leaves of Prunus domestica L. has been column chromatographed using gradient of ethyl acetate in petroleum ether as solvent. This has yielded a red coloured fraction/band (RCF) (approximately 130 mg), which has further been analysed by GC-MS for the identification of bioactive phytoconstituents. The RCF has been analysed for antioxidant activity by DPPH method which has shown an IC50 value of 43.895 mg/mL. The antibacterial activity of RCF has been assessed by agar well diffusion method against E. coli which has shown the zone of inhibition of 17 mm. Stigmast-5-en-3-ol, oleate (31.77%) is identified as the major phytoconstituent and molecular docking of this major constituent has been carried out to observe its interactions with heme oxygenase and DNA gyrase protein chains.
 
Date 2024-07-19T07:13:43Z
2024-07-19T07:13:43Z
2024-07
 
Type Article
 
Identifier 2583-1321 (Online); 0019-5103 (Print)
http://nopr.niscpr.res.in/handle/123456789/64260
https://doi.org/10.56042/ijc.v63i7.7567
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJC Vol.63(07) [July 2024]