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Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India

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Title Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India
Not Available
 
Creator Vineeth Rajan
Ardhra Vijayan
Ambili P. M.
Justus Lancy
Sivaraman, G. K.
 
Subject Acinetobacter baumannii
Biofilm formation
Carbapenem resistance
Disinfectant resistance
Pulsed-field gel electrophoresis
 
Description Not Available
Acinetobacter baumannii poses a significant challenge in healthcare settings across the globe, with isolates exhibiting carbap enem resistance at unprecedented rates. Here, we characterized a collection of A. baumannii isolates (n=64) recovered during
the period September 2020 – November 2021 at a teaching hospital in Cochin, South India. The species identity of the isolates
was confirmed with blaOXA-51-like PCR. The major carbapenemase determinants identified were blaOXA-23-like (45, 70.3%) and blaNDM-1
(31, 48.4%); co-occurrence of these genes was also observed in 27 (42.2%) isolates. Other resistance genes identified included
blaPER (34, 53.1%), aac(6')-Ib-cr (42, 65.6%), qnrS (25, 39.1%), sul1 (32, 50%), sul2 (33, 51.6%), strA/strB (36, 56.3%), aphA1-Iab (35,
54.7%) and tetB (32, 50%). Mapping PCR revealed the insertion element, ISAbaI upstream of blaOXA-23-like in all isolates possessing
this gene. Concerning disinfectant resistance, all isolates carried the quaternary ammonium compound (QAC) resistance gene,
qacEΔ1. Minimal inhibitory concentration (MIC) of benzalkonium chloride was high among the isolates and ranged from 8 to
128µgml−1. However, low MICs were observed for chlorhexidine and triclosan, with the majority (54, 80.6%) of isolates showing
an MIC of 2µgml−1 for chlorhexidine and all isolates exhibiting MICs of ≤0.125µgml−1 for triclosan. Further, all isolates were
strong biofilm-producers, as assessed by the crystal violet-based microtitre plate assay. The ApaI-pulsed-field gel electropho resis (PFGE) revealed the multi-clonal nature of the isolates, with 16 clusters and 16 unique pulsotypes identified at a cut-off of
80%. In short, this study provides useful data on the molecular features of A. baumannii from this region, which could be helpful
to assess the local epidemiology of this pathogen and also to devise infection control strategies.
Not Available
 
Date 2024-07-06T20:27:24Z
2024-07-06T20:27:24Z
2023-07-04
 
Type Research Paper
 
Identifier Vineeth Rajan, Ardhra Vijayan, Ambili P. M., Justus Lancy and Sivaraman, G. K. (2023) Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India. Access Microbiology, 5(11): 1-20.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/83876
 
Language English
 
Relation Not Available;
 
Publisher Microbiology Society