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Molecular detection of Brucella melitensis in sheep from district Budgam, Jammu and Kashmir

Indian Agricultural Research Journals

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Title Molecular detection of Brucella melitensis in sheep from district Budgam, Jammu and Kashmir
 
Creator BASHIR, KHALID
HUSSAIN, TUFAIL
QADRI, NOUSHEEN
BABA, JAVAID AHMAD
SHAH, IMRAN HAMID
RATHER, MUBASHIR ALI
 
Subject Abortion, Brucella melitensis, PCR, Sheep, Zoonotic
 
Description Farmers reported abortions and stillbirths in 600 sheep including 471 pregnant ewes. A complete investigation including physical examination of livestock, postmortem examination of stillbirths, serological examination of ewes and molecular study of abomasal contents, peritoneal fluid, liver and kidney tissue collected from still born lambs was done to ascertain the cause of mortality at Disease Investigation Laboratory, Nowshera, Srinagar. Also, on spot heat fixed tissue smears were prepared for Gram’s staining and accordingly observed for presence of organism. Extraction of bacterial genomic DNA from samples from still born lambs was performed and obtained DNA extracts were checked by agarose gel electrophoresis and stored at -20°C till further use. The PCR conducted on the abomasal, peritoneal contents and liver and kidney tissues revealed 731 bp band after the gel electrophoresis, confirming Brucella melitensis as cause of stillbirths in the flock. The investigation revealed that Brucella melitensis was cause of stillbirths and abortions in sheep flocks.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-08-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/151333
10.56093/ijans.v94i8.151333
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 8 (2024); 678–680
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/151333/55359
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0