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Isolation of Lactobacilli from Malabari Goat Milk Samples and Assessment of their Techno-Functional Attributes for use as Functional Starter Cultures

Indian Agricultural Research Journals

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Title Isolation of Lactobacilli from Malabari Goat Milk Samples and Assessment of their Techno-Functional Attributes for use as Functional Starter Cultures
 
Creator Amrutha, T.A
Ligimol James
A. K. Beena
S. N.Rajakumar
Aparna Sudhakaran, V
Divya, M.P
 
Subject Malabari goat milk, Lactiplantibacillus plantarum, Functional starter cultures
 
Description The objective of this study was to isolate lactobacilli from raw goat milk samples and characterize them in terms of their techno-functional attributes. Out of the eleven distinct colonies obtained on pour plating five individual goat milk samples three isolates were tentatively identified as lactobacilli cultures. Through 16S rRNA sequencing these isolates were identified as  Lactiplantibacillus plantarum DMAG02, Lactiplantibacillus plantarum  DMAG03, and Lactiplantibacillus plantarum  DMA11, and their partial 16S rRNA sequences were deposited in NCBI with accession nos:  OR105028, OR105041   and OR905856. In-vitro studies revealed the antimicrobial activity of these Lactiplantibacillus plantarum strains towards Staphylococcus aureus. Out of the isolates, DMA11 alone was found to be citrate fermenting and positive for Voges Proskauer and methyl red reaction. All the isolates showed proteolytic activity, while none of them exhibited lipolytic activity and exopolysaccharide production. Out of three isolates, DMA11 exhibited the highest cell surface hydrophobicity (93.34%), auto aggregation  (88.5%), and DPPH scavenging activity (31.33%). In the case of antibiotic susceptibility, all three isolates exhibited sensitivity against three (Azythromycin, Tetracycline, and Clindamycin) out of the seven tested antibiotics. The safety assessment tests revealed that all the isolates are non-virulent. In light of the safety features, functional qualities, and technological traits of the isolated Lactiplantibacillus strains, they hold promise for usage as functional dairy starter cultures.
 
Publisher Indian Veterinary Association
 
Date 2024-08-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IVJ/article/view/154775
10.62757/
 
Source The Indian Veterinary Journal; Vol. 101 No. 07 (2024): July, 2024; 27 - 38
0974-9365
0019-6479
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IVJ/article/view/154775/55387
 
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