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GENOTYPING OF HOLSTEIN FRIESIAN CROSSBRED CATTLE FOR CVM AND FXI DEFICIENCY LOCI

Indian Agricultural Research Journals

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Title GENOTYPING OF HOLSTEIN FRIESIAN CROSSBRED CATTLE FOR CVM AND FXI DEFICIENCY LOCI
 
Creator S.B. Khade
V.D. Pawar
A.Y. Doiphode
M.P. Sawane
U.D. Umrikar
 
Subject CVM
FXID
PCR
PCR-RFLP
 
Description In animal breeding, genetic disorders are one of the most important issues for breeders. The present study was carried out to genotype HF crossbred cattle for FXI deficiency and CVM using PCR and PCR-RFLP using 50 blood samples. Amplicons of 244 bp for FXI gene and the 287 bp for SLC35A3 gene were successfully amplified. Analysis of amplicons of FXI gene revealed single 244 bp fragment which indicates absence of mutant allele. The amplicons of 287 bp of SLC35A3 gene was digested with Pst I RE which revealed two fragments of size 264 bp and 23 bp  for normal animals. In the present study, out of fifty HF crossbred animals screened neither carrier nor affected animals were found for FXID and CVM.  
 
Publisher Tamil Nadu Veterinary and Animal Sciences University
 
Date 2024-08-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJVASR/article/view/153443
10.56093/ijvasr.v43i5.153443
 
Source Indian Journal of Veterinary and Animal Sciences Research; Vol. 43 No. 5 (2014): IJVASR Sep - Oct 2014; 340-345
2347-2774
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJVASR/article/view/153443/55052
 
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