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CLONING AND EXPRESSION OF ENDOGLUCANASE GENE FROM TERMITE GUT BACTERIA FOR IMPROVING FIBRE DIGESTION *

Indian Agricultural Research Journals

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Title CLONING AND EXPRESSION OF ENDOGLUCANASE GENE FROM TERMITE GUT BACTERIA FOR IMPROVING FIBRE DIGESTION *
 
Creator N.V. Aruna
K.Vijayarani
 
Subject Odontotermes formosanus
Salmonella enterica
Endoglucanase gene
Cloning and expression
 
Description The present study describes the cloning and expression of endoglucanase gene from termite gut bacteria. Salmonella enterica with cellulolytic activity was  isolated from the gut of the termite Odontotermes formosanus and characterized by sugar fermentation tests, biochemical tests and 16S rRNA  sequencing. Endoglucanase gene was amplified from the genomic DNA of the bacteria and the purified 1.72 Kbp Polymerase chain reaction product was cloned into pET100 prokaryotic vector. The recombinant plasmid expressed a 65kDa endoglucanase which reacted with anti His-HRP antibody in western blotting. Cellulolytic activity of the recombinant plasmid was confirmed by congo red assay and the enzyme activity was found to be 54 Units/ml. 
 
Publisher Tamil Nadu Veterinary and Animal Sciences University
 
Date 2024-08-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJVASR/article/view/153648
10.56093/ijvasr.v43i6.153648
 
Source Indian Journal of Veterinary and Animal Sciences Research; Vol. 43 No. 6 (2014): IJVASR Nov - Dec 2014; 393-398
2347-2774
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJVASR/article/view/153648/55089
 
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