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ANTI-BACTERIAL ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED USING PHYLLANTHUS AMARUS AQUEOUS & ANDROGRAPHIS PANICULATA ETHANOLIC EXTRACTS

Indian Agricultural Research Journals

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Title ANTI-BACTERIAL ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED USING PHYLLANTHUS AMARUS AQUEOUS & ANDROGRAPHIS PANICULATA ETHANOLIC EXTRACTS
 
Creator M. Ananda Chitra
S. Ramesh
 
Subject Silver nanoparticles
antibacterial activity
P.amarus
A.paniculata
green synthesis
 
Description The use of metallic silver as an antimicrobial agent has been recognized for centuries. Silver nanoparticles (SNP) are now incorporated in apparel, wound dressings, appliances, cosmetics, paints and plastics for their antimicrobial properties. Generally, silver nanoparticles are prepared by a variety of chemical methods. In this study, we have used Phyllanthus amarus and Andrographis paniculata herbal plants leaves and stem extracts to synthesis SNP and were characterized by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). SNP capped with plant extracts gave absorption peak at 420 nm as expected for silver and broadening of peak were also noticed. TEM images suggested that they were of almost spherical shape and in the range of 7-60 nm in size. Antibacterial activity of plant extracts capped SNP were tested in Mueller-Hinton agar by well diffusion method against Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, Escherichia coli and Salmonella Typhimurium.  SNP of 1 mg/ml and 5 mg/ml of P.amarus exhibited almost similar antibacterial activity, whereas, 5mg/ml, not 1 mg/ml of SNP capped with A. paniculata exerted antibacterial activity. The highest zone of inhibition was noticed against B.subtilis followed by S.aureus and P.vulgaris and the least zone of inhibition was observed against E.coli and S.Typhimurium. Green synthesis of SNP is cost effective and environment friendly. Further studies are required to explore the possibility of use of P.amarus capped silver nanoparticles for the treatment of burn and wound.
 
Publisher Tamil Nadu Veterinary and Animal Sciences University
 
Date 2024-08-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJVASR/article/view/147706
10.56093/ijvasr.v44i1.147706
 
Source Indian Journal of Veterinary and Animal Sciences Research; Vol. 44 No. 1 (2015): IJVASR January - February 2015; 12-19
2347-2774
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJVASR/article/view/147706/53696
 
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