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EFFECT OF GLUTATHIONE ON SEMINAL QUALITY PARAMETERS AT EQUILIBRATION IN SURTI BUFFALO BULL

Indian Agricultural Research Journals

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Title EFFECT OF GLUTATHIONE ON SEMINAL QUALITY PARAMETERS AT EQUILIBRATION IN SURTI BUFFALO BULL
 
Creator Kailash Kumar
Mitesh Gaur
 
Subject Glutathione
Pre-freeze quality
Surti buffalo
Bubalus bubalis
Semen additive
 
Description The main aim of this study was to investigate the effect of different concentrations of glutathione supplementation on liquid storage of Surti buffalo bull semen. This study was performed on adult Surti buffalo bull (n = 6), and seminal ejaculates (24) were collected and evaluated for various microscopic seminal quality parameters for further processing. After preliminary evaluation, ejaculates of each collection session were mixed and divided into four equal aliquots. All the aliquots were diluted (1:10) with Tris fructose egg yolk citrate extender contained glutathione served as control (T0), whereas the other three aliquots were supplemented with 0.5, 2.0 and 5.0 mM glutathione which were grouped as Treatment-1 (T1), Treatment-2 (T2) and Treatment-3 (T3), respectively. Thereafter, the samples were stored at 4 ºC for 4 h, and various seminal parameters (individual sperm progressive motility, viability, abnormalities, plasma membrane functionality) were evaluated at equilibration. The results indicated that the mean percent values for pre-freeze sperm progressive motility, live sperm percentage and HOS responsive spermatozoa were found to be significantly higher (P<0.05) (except individual progressive motility which was non-significantly higher in Treatment-3); whereas sperm abnormalities were significantly lower (P<0.05) in semen samples treated with glutathione (Treatment-1, Treatment-2 and Treatment-3) in comparison to control. The Treatment-2 (2.0 mM glutathione), had the highest pre-freeze sperm progressive motility percentage, live sperm percentage, HOS response sperm percentage and reduced sperm abnormalities percentage compared to other three groups. 
 
Publisher Tamil Nadu Veterinary and Animal Sciences University
 
Date 2024-08-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJVASR/article/view/154855
10.56093/ijvasr.v53i3.154855
 
Source Indian Journal of Veterinary and Animal Sciences Research; Vol. 53 No. 3 (2024): IJVASR May - June 2024; 21-30
2347-2774
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJVASR/article/view/154855/55429
 
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