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Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa

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Relation http://oar.icrisat.org/1313/
http://dx.doi.org/10.1111/j.1467-7652.2011.00625.x
 
Title Large-scale transcriptome analysis in chickpea (Cicer
arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa
 
Creator Hiremath, P J
Farmer, A
Cannon, S B
Woodward, J
Kudapa, H
Tuteja, R
Kumar, A
Bhanuprakash, A
Mulaosmanovic, B
Gujaria, N
Krishnamurthy, L
Gaur, P M
Kavi Kishor, P B
Shah, T
Srinivasan, R
Lohse, M
Xiao, Y
Town, C D
Cook, D R
May, G D
Varshney, R K
 
Subject Chickpea
 
Description Chickpea (Cicer arietinum L.) is an important legume crop in the semi-arid regions of Asia and Africa. Gains in crop productivity have been low however, particularly because of biotic and abiotic stresses. To help enhance crop productivity using molecular breeding techniques, next generation sequencing technologies such as Roche/454 and Illumina/Solexa were used to determine the sequence of most gene transcripts and to identify drought-responsive genes and gene-based molecular markers. A total of 103 215 tentative unique sequences (TUSs) have been produced from 435 018 Roche/454 reads and 21 491 Sanger expressed sequence tags (ESTs). Putative functions were determined for 49 437 (47.8%) of the TUSs, and gene ontology assignments were determined for 20 634 (41.7%) of the TUSs. Comparison of the chickpea TUSs with the Medicago truncatula genome assembly (Mt 3.5.1 build) resulted in 42 141 aligned TUSs with putative gene structures (including 39 281 predicted intron/splice junctions). Alignment of ∼37 million Illumina/Solexa tags generated from drought-challenged root tissues of two chickpea genotypes against the TUSs identified 44 639 differentially expressed TUSs. The TUSs were also used to identify a diverse set of markers, including 728 simple sequence repeats (SSRs), 495 single nucleotide polymorphisms (SNPs), 387 conserved orthologous sequence (COS) markers, and 2088 intron-spanning region (ISR) markers. This resource will be useful for basic and applied research for genome analysis and crop improvement in chickpea.
 
Publisher Blackwell Publishing
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1313/1/PlantBiotechnolJou9_8_922-931-2011.pdf
Hiremath, P J and Farmer, A and Cannon, S B and Woodward, J and Kudapa, H and Tuteja, R and Kumar, A and Bhanuprakash, A and Mulaosmanovic, B and Gujaria, N and Krishnamurthy, L and Gaur, P M and Kavi Kishor, P B and Shah, T and Srinivasan, R and Lohse, M and Xiao, Y and Town, C D and Cook, D R and May, G D and Varshney, R K (2011) Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa. Plant Biotechnology Journal, 9. pp. 922-931.