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Genetic diversity of Colletotrichum graminicola isolates from India revealed by restriction analysis of PCR-amplified intergenic spacer region of nuclear rDNA

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Title Genetic diversity of Colletotrichum graminicola isolates from India revealed by restriction analysis of PCR-amplified intergenic spacer region of nuclear rDNA
 
Creator Latha, J
Chakrabarti, A
Mathur, K
Rao, V P
Thakur, R P
Mukherjee, P K
 
Subject Agriculture-Farming, Production, Technology, Economics
 
Description Sorghum anthracnose, caused by Colletotrichum graminicola (Ces.) Wilson, is a destructive disease responsible for as
high as 50% loss in grain yield1. Management of this disease through host plant resistance has often been unsuccessful due to the hyper-variable nature of this
fungus2. A rapid and reproducible tool for characterizing the pathogen genotypes would help researchers follow the
shift in genetic make-up of the pathogen population, thus providing a dynamic picture of the interactions between the
host and pathogen genotypes. This would, in turn, help devising strategies for management of this disease. Genetic
variability in this fungus was earlier studied by using molecular tools like RFLP and RAPD3–7. RFLP is a reliable tool, but is cumbersome, time-consuming and requires large amount of DNA. RAPD,on the other hand, is simple and rapid,
but often not reproducible and errorprone8. Restriction analysis of the intergenic spacer region of the rDNA repeats
has been useful for variability studies in some fungi like Fusarium oxysporum9–11 and Pyrenophora graminea12. Once optimized (primer sequences and enzyme combinations), this technique combines the advantage of both PCR (simplicity
and speed) and RFLP (reproducibility). The present communication reports on the successful use of the primer pair originally designed for F. oxysporum9 and identification of a single restriction enzyme, KpnI, which can be used for fingerprinting of C. graminicola populations.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1331/1/CurrSci84_7_881-883_2003.pdf
Latha, J and Chakrabarti, A and Mathur, K and Rao, V P and Thakur, R P and Mukherjee, P K (2003) Genetic diversity of Colletotrichum graminicola isolates from India revealed by restriction analysis of PCR-amplified intergenic spacer region of nuclear rDNA. Current Science, 84 (7). pp. 881-883.