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Combined Effect of Two Transgenes by Host Delivered RNAi against Meloidogyne incognita and its Parasitic Potential

Indian Agricultural Research Journals

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Title Combined Effect of Two Transgenes by Host Delivered RNAi against Meloidogyne incognita and its Parasitic Potential
 
Creator GANGWAR, SACHIN
SIROHI , ANIL
 
Subject Arabidopsis, dsRNA, Meloidogyne incognita, PPN, RKN, RNAi, siRNA
 
Description In the current situation, the most serious threat to agriculture is root-knot nematodes (RKNs), which cause both direct and indirect damage. RKNs (Meloidogyne incognita) are obligatory sedentary plant endoparasites with a highly complicated parasitism strategy. Plant resistance development is a promising approach to dealing with the RKN problem. RNA interference (RNAi) is a revolutionary way for engineering plants to create resistance against plant parasites and pathogens. Splicing factor and integrase are two housekeeping genes in Meloidogyne incognita which were targeted through RNAi for silencing. The target organism (M. incognita) was subjected to host-induced gene silencing (HIGs) of these two genes at the same time by crossing parents that had already been altered to express the dsRNA of each gene independently. Bioassay tests were performed on the progeny that expressed dsRNA for both genes. The findings revealed a significant reduction in the pathogenic and reproductive capability of M. incognita. When compared to untransformed occurrences, the number of galls, females, and egg masses were reduced by 71.81-74.39, 76.73-78.76, and 67.13-70.27 per cent, respectively. The results demonstrated that combinatorial gene silencing had a greater effect than single-gene silencing or no gene silencing at all.
 
Publisher Nematological Society of India
 
Date 2024-09-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJN/article/view/156045
 
Source Indian Journal of Nematology; Vol. 53 No. 1 (2023): Indian Journal of Nematology; 21-27
0974-4444
0303-6960
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJN/article/view/156045/55694
 
Rights Copyright (c) 2024 Indian Journal of Nematology
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