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Effect of lyophilized heterologous seminal plasma supplementation on Marwari stallion epididymal spermatozoa during 24 hours cool storage

Indian Agricultural Research Journals

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Title Effect of lyophilized heterologous seminal plasma supplementation on Marwari stallion epididymal spermatozoa during 24 hours cool storage
 
Creator RAM, VACHNA
JHAMB, DINESH
GAUR, MITESH
KUMAR, NAVEEN
TALLURI, THIRUMALA RAO
SOLANKI, OMPRAKASH
JUNEJA, ROHIT
PARGI, KALPESH KUMAR
NANDAN, DEVKI
ARORA, SHARAD
VYAS, HIMANSHU
 
Subject Castration, Cool sperm, Equine, Epididymal sperm, HOST, Seminal plasma
 
Description A study was carried out to investigate the effect of storage of testes-epididymis complex at 5oC for 24 h and supplementation of lyophilized heterologous seminal plasma (LHSP) at different concentrations on the sperm quality during cool storage. Six pairs of testicles were collected immediately after orchiectomy from six stallions. One testes-epididymis (F1) was immediately processed for sperm retrieval and second testes-epididymis (F2) complex of each stallion was stored at 5℃ for 24 h. Spermatozoa were harvested from the epididymis by flotation technique and evaluated. Highly significant difference was observed among mean values of progressive motility (%) of epididymal sperm after 0 and 24 h of testicular-epididymal complex storage at 5°C, while no significant difference was observed among mean values of live sperm, membrane integrity and acrosomal integrity (%). Both epididymal sperm suspensions were supplemented with skim milk glucose semen extender fortified with different concentrations (0, 2, 5 and 10 mg/ml) of LHSP and cooled for 24 h. Sperm suspension evaluation at 0, 12 and 24 h of cool storage revealed that addition of 2 mg/ml LHSP has beneficial effect as compared to control on progressive motility and HOST in both epididymal sperm suspension at 12 h and 24 h of cool storage at 5℃. From the current study, it was inferred that the sperm quality was not deteriorated even after the sperm retrieval after 24 h from the epididymis on storage at 5℃. Further, LHSP supplemented @2 mg/ml originated from good fertility stallion is the suitable concentration for cool-storage of equine epididymal sperm suspension.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-09-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/145538
10.56093/ijans.v94i9.145538
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 9 (2024); 786–791
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/145538/55731
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0