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Isolation and characterization of exosomes derived from buffalo oviductal epithelial cells

Indian Agricultural Research Journals

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Field Value
 
Title Isolation and characterization of exosomes derived from buffalo oviductal epithelial cells
 
Creator K, NAHEEF
K, BRINDHA
M A, SENTHIL KUMAR T
S L, SESH P
M, PARTHIBAN
S, RANGASAMY
 
Subject Exosomes, In vitro culture, Oviductal epithelial cells (OECS)
 
Description In the present study, exosomes were successfully isolated from the conditioned medium of buffalo OECs cultured in vitro. Characterisation by nanoparticle analysis revealed that the size of the oviductal exosomes ranged between 40-150 nm. Imaging by Transmission electron microscopy showed that these exosomes exhibited circular/spherical morphology. The identity of isolated exosomes was further confirmed by analysing the expression of surface expressed tetraspannin markers (CD9, CD63, and CD81) by flow cytometry, and were found to be enriched with the tetraspannins analysed. As continuation of this work, further research to supplement the isolated oviductal exosomes to recreate the preimplantation physiological environment of the oviduct should be undertaken, thus improving in vitro culture conditions for buffalo embryos.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-09-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/148385
10.56093/ijans.v94i9.148385
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 9 (2024); 770–773
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/148385/55726
https://epubs.icar.org.in/index.php/IJAnS/article/view/148385/55727
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0