Record Details

Understanding the effect of carbon dots with size variation on collagen

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Understanding the effect of carbon dots with size variation on collagen
 
Creator M, Renuka
V, Harini
Balasubramanian, Surabhya
J, Sridevi
V G, Vaidyanathan
 
Subject Carbon dots
Fibrillation
Photoirradiation
Radicals
 
Description 791-796
The unique optical properties of carbon dots (CDs) offer a wide variety of applications both in chemical and biological
environments. The interaction of CDs with biological molecules such as proteins and DNA has been studied extensively. In
the present study, the interaction of two different sizes of CDs with collagen, a fibrillar protein, has been investigated. The
different sizes of CDs have been characterized using various techniques such as HR-TEM, SEM, and zeta potential. Upon
interaction with CDs, the triple helical nature was not altered and Rpn value was similar to the control collagen. The
influence of size on the fibrillation of collagen process is significantly impacted with photoirradiation process. Similarly, in
DSC experiments, the CDs destabilized the collagen with Tm value of 161C but with photoirradiation, the stability of
the collagen is improved but less than the control collagen. In EPR studies upon photoirradiation, a significant amount of
generation of hydroxyl radicals is observed. The gel electrophoresis studies show that the presence of CDs did not inhibit the
collagenase activity without or with photoirradiation. The data from the present study clearly reveals that the carbon dots
can be utilized with varied drug loading on collagen that can be utilized for bioengineering applications.
 
Date 2024-08-21T05:09:35Z
2024-08-21T05:09:35Z
2024-08
 
Type Article
 
Identifier 2583-1321 (Online); 0019-5103 (Print)
http://nopr.niscpr.res.in/handle/123456789/64417
https://doi.org/10.56042/ijc.v63i8.8764
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJC Vol.63(08) [August 2024]