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Determination of stress levels of Otolithoides biauritus (Cantor, 1849) in selected zones of Indian Sundarbans

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Title Determination of stress levels of Otolithoides biauritus (Cantor, 1849) in selected zones of Indian Sundarbans
 
Creator Mitra, Jhinook
Mondal (Parui), Sanjukta
 
Subject Aquatic pollution
Cortisol
Hsp70
 
Description 693-699
The Indian Sundarbans is one of the three largest single tract
mangrove forests of the world. The hydro-geochemical
environment of this ecosystem is highly dynamic. Factors such as
global warming, heavy siltation and solid waste disposal have
caused considerable damage to the ecological status of the Indian
Sundarbans and also affected the natural fish population. Here, we
determined the stress levels of the amphidromous Sciaenid
Otolithoides biauritus (Cantor, 1849) in response to water quality
parameters of selected zones of Indian Sundarbans which can
indicate its capacity to survive environmental stress by
potentiating stress response through molecular activities. Samples
were collected during the post-monsoon season in February 2023
from four stations namely S1 (22°09.943'N 088°53.325'E), S2
(22°09.661'N 088°54.948'E), S3 (22°00.857'N 088°45.567'E) and
S4 (21°34.861'N 088°14.157'E) with different salinity regimes
and the complete protein profile of muscles was established by
SDS-PAGE. The concentrations of stress protein Hsp70 (pg/mL)
and cortisol hormone (ng/mL) were determined via quantitative
ELISA method. Physicochemical parameters such as water
temperature, salinity, pH, dissolved oxygen, total dissolved solids
(TDS), transparency and depth were recorded in situ. Nutrients
such as nitrate, phosphate and silicate concentrations were
estimated via standard spectrophotometric methods. A
comparative account of the findings was established between S1,
S2, S3 and S4. Highest average cortisol concentrations were
observed at S1, while highest Hsp70 at S3. A 97 kDa protein was
found exclusively at S2 while two proteins (26 kDa and 23.5 kDa)
found at S1, S3 and S4, were absent at S2. S2 recorded least
cortisol and Hsp70 levels. A long term monitoring is
recommended for better understanding of the spatial variation of
protein profile under fluctuating hydrodynamic conditions of the
Indian Sundarbans.
 
Date 2024-09-09T05:24:43Z
2024-09-09T05:24:43Z
2024-09
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64481
https://doi.org/10.56042/ijeb.v62i08.6680
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(08) [August 2024]