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Spectrophotometric quantification of biotin in drug formulations utilizing Pd(II) promoted ligand substitution approach in micellar medium

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Title Spectrophotometric quantification of biotin in drug formulations utilizing Pd(II) promoted ligand substitution approach in micellar medium
 
Creator Srivastava, Abhishek
Srivastava, Neetu
Singh, Vinay Kumar
 
Subject Biotin determination
Hexacyanoferrate(II)
Inhibitory approach
Kinetic-spectrophotometric
Ligand substitution reaction
Pharmaceutical preparations
 
Description 691-701
A spectrophotometric approach that is straightforward, efficient, highly sensitive, and precise has been devised for
quantifying biotin in both its pure state and pharmaceutical samples. Analysis of biotin in biological and pharmaceutical
samples is essential for therapeutic evaluation and patient follow-up bioavailability. Several methods for determining this
drug have drawbacks including specialized equipment that many quality control laboratories and universities in developing
countries lack. The methodology relies on the inhibitory approach of biotin on the Pd(II) promoted ligand substitution (LS)
reaction involving phenylhydrazine (PHZ) and hexacyanoferrate(II). The process entails replacing cyanide in [Fe(CN)6]4-
with PHZ, triggering the development of a complex [Fe(CN)5PHZ]3-. The complex demonstrates a significant level of
absorption at a specific wavelength of 488 nm. The established limit of detection for biotin is 0.117 μg mL−1. Experiments
on recovery are conducted to confirm the precision and accuracy of biotin quantification. The suggested approach has been
effectively utilized for the examination of biotin in pristine samples and various medications, demonstrating remarkable
levels of precision and accuracy. The outcomes show good agreement when compared to the findings of the official
analytical method. The excipients typically employed in medicines do not exhibit any interference with the suggested
methodology. This methodology is highly effective for accurately determining trace levels of different drugs and biological
molecules that can significantly impede the catalytic efficiency of Pd(II).
 
Date 2024-09-13T11:54:53Z
2024-09-13T11:54:53Z
2024-09
 
Type Article
 
Identifier 0975-0991 (Online); 0971-457X (Print)
http://nopr.niscpr.res.in/handle/123456789/64536
https://doi.org/10.56042/ijct.v31i5.10906
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJCT Vol.31(5) [September 2024]