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Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA

OAR@ICRISAT

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Relation http://oar.icrisat.org/1513/
http://dx.doi.org/10.1023/A:1015759318497
 
Title Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA
 
Creator Sivaramakrishnan, S
Kannan, S
Reddy, L J
 
Subject Pigeonpea
 
Description Diversity in 28 accessions representing 12 species of the genus, Cajanus arranged in 6 sections including 5 accessions of the cultivated species, C. cajan, and 4 species of the genus Rhyncosia available in the germplasm collection at ICRISAT was assessed using RFLP with maize mtDNA probes. Cluster analysis of the Southern blot hybridization data with 3 restriction enzymes – 3 probe combinations placed the genus Rhyncosia in a major group well separated from all the species belonging to the genus Cajanus.Within the genus Cajanus, the 4 accessions of C. platycarpus belonging to section Rhynchosoides formed a separate group in contrast to those in other sections of pigeonpea. In the section, Cajanus all the 5 accessions of C. cajan were grouped together and C. cajanifolius belonging to the same section was in a subgroup by itself closer to the main group. The four accessions of C. scarabaeoides, were together and the other species belonging to section Cantharospermum were in different subgroups. The intra-specific variation was seen even within accessions of certain pigeonpea wild species such as C. scarabaeoides, C. platycarpus, C. acutifolius, and even the cultivated species of C. cajan. This study suggests that RFLP of mtDNA can be used for the diversity analysis of pigeonpea and it gives some indications on the maternal lineage among the species. The variations in the mitochondrial DNA hybridization patterns also suggest the extensive rearrangement of the organelle genome among the Cajanus species.
 
Publisher Kluwer
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1513/1/Euphytica125_1_21-28_2002.pdf
Sivaramakrishnan, S and Kannan, S and Reddy, L J (2002) Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA. Euphytica, 125 (1). pp. 21-28.