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Differentiation of Eastern and Western Strains of Peanut Stunt Cucumovirus Based on Satellite RNA Support and Nucleotide Sequence Homology

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Relation http://oar.icrisat.org/1528/
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1995Abstracts/Phyto_85_502.htm
 
Title Differentiation of Eastern and Western Strains of Peanut Stunt Cucumovirus Based on Satellite RNA Support and Nucleotide Sequence Homology
 
Creator Naidu, R A
Hu, C C
Pennington, R E
Ghabrial, S A
 
Subject Agriculture-Farming, Production, Technology, Economics
 
Description Two western strains of peanut stunt cucumovirus (PSV), strains W and BV-15, were unable to support the replication of 2 distinct PSV satellite RNAs (satRNAs), or infectious transcripts from full-length satRNA cDNA clones, in either tobacco or several leguminous host species. These 2 strains also did not support the replication of cucumber mosaic cucumovirus satRNAs in tobacco. However, all 10 eastern PSV strains tested efficiently supported PSV satRNA replication. Strains PSV-W and BV-15 can be further differentiated from the eastern strain PSV-ER by Northern hybridization using cloned cDNA probes to PSV-ER RNAs 1, 2 and 3. Whereas the PSV-ER RNA-specific probes did not hybridize to any PSV-W RNAs, the PSV-ER RNA 3 probe hybridized strongly to PSV-BV-15 RNAs 3 and 4, supporting the finding that strain BV-15 is serologically closely related to the eastern strains. The PSV-ER RNA 2-specific probe, but not the PSV-ER RNA 1-specific probe, hybridized to the respective RNA of PSV-BV-15. The results of Northern hybridization with strain PSV-BV-15 support the contention that strain BV-15 represents a reassortant between western and eastern strains. The lack of cross-hybridization between PSV-ER and PSV-W RNAs in Northern hybridization at high stringency is consistent with finding that the percentages of nucleotide identity between the RNAs of strains PSV-ER and PSV-W were 75, 73, 74 and 74%, respectively, for RNAs 1, 2, 3 and 4. A procedure based on reverse transcription and the polymerase chain reaction (RT-PCR) was developed that utilized primer specific for PSV-ER RNA 2. Restriction enzyme digestion of the RT-PCR products generated distinct restriction patterns that clearly differentiated western from eastern strains.
 
Date 1995
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1528/1/JA_2207.pdf
Naidu, R A and Hu, C C and Pennington, R E and Ghabrial, S A (1995) Differentiation of Eastern and Western Strains of Peanut Stunt Cucumovirus Based on Satellite RNA Support and Nucleotide Sequence Homology. Journal Phytopathology, 85 (4). pp. 502-507.