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Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta)

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Relation http://oar.icrisat.org/1542/
http://dx.doi.org/10.1139/G02-045
 
Title Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta)
 
Creator Mace, E S
Godwin, I D
 
Subject Genetics and Genomics
 
Description Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection.
 
Publisher National Research Council Canada
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1542/1/Genome45_5_823-832_2002.pdf
Mace, E S and Godwin, I D (2002) Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta). Genome, 45 (5). pp. 823-832. ISSN 1480-3321