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Method validation for the detection of formaldehydein fish using ultra performance liquid chromatography

Indian Agricultural Research Journals

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Title Method validation for the detection of formaldehydein fish using ultra performance liquid chromatography
 
Creator Dhruve, Domendra
Rajendran Shalini
Robinson Jeya Shakila
Ulaganthan Arisekar
Geevaretnam Jayasekaran
Asha Hemamalini
Vedprakash Ratrey
 
Subject Method validation, UPLC, formaldehyde, fish, formalin abuse
 
Description Formaldehyde, the simplest aldehyde, tops the list of illegal and harmful food contaminants. Recently, formaldehyde has been used indiscriminately to preserve the freshness and quality of fish. Numerous incidents of formalin (a solution of formaldehyde) misuse during the storage and transportation of fish have been reported in several parts of India. In this context, it is pertinent to study formalin contamination in fish and shellfish. Protocol for detection of free and bound formaldehyde by ultra-performance liquid chromatography (UPLC) was attempted in the present study. Formaldehyde peak was observed in the expected retentiontime and standardisation of the protocol for formaldehyde determination was then carried out. UPLC method was developed and validated for specificity, repeatability, accuracy, and linearity. The average correlation coefficient (R2) was 0.99. The recovery rates of free and bound formaldehyde were 90.83 to 116.43% and 88.76 to 132.98%, respectively. The limit of detection (LOD) was found to be 0.5 ppm. The study investigated free and bound formaldehyde levels in commonly traded fish species across five major fish markets inTamil Nadu, India. Sphyraena barracuda had the highest recorded concentrations of free and bound formaldehyde at 9.8 and 6.45 mg kg-1, respectively, while Lethrinus lentjan exhibited the lowest concentrations at 0.8 mg kg-1 for free formaldehyde and 0.5 mg kg-1 for bound formaldehyde.
 
Keywords: Formalin abuse, Limit of detection, Method validation, Retention time UPLC
 
Publisher Central Marine Fisheries Research Institute (on behalf of Indian Council of Agricultural
 
Date 2024-09-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJF/article/view/139843
10.21077/ijf.2024.71.3.139843-13
 
Source Indian Journal of Fisheries; Vol. 71 No. 3 (2024)
0970-6011
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJF/article/view/139843/56046
 
Rights Copyright (c) 2024 Indian Journal of Fisheries
https://creativecommons.org/licenses/by-nd/4.0