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The nucleotide sequence of RNA-1 of Indian peanut clump furovius

OAR@ICRISAT

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Relation http://oar.icrisat.org/1574/
http://dx.doi.org/10.1007/BF01718632
 
Title The nucleotide sequence of RNA-1 of Indian peanut clump furovius
 
Creator Miller, J S
Wesley, S V
Naidu, R A
Reddy, D V R
Mayo, M A
 
Subject Agriculture-Farming, Production, Technology, Economics
 
Description The nucleotide sequence of RNA-1 of an isolate of the H serotype of Indian peanut clump virus (IPCV-H) was shown to comprise 5 841 nucleotides. The RNA contains three open reading frames (ORF) which are between nucleo¬tides 133 and 3522, nucleotides 3526 and 5103 (assuming expression by suppression of the ORF 1 termination codon) and nucleotides 5 168 and 5 539. The encoded polypeptides have M,, of 129687 (pl30), 60188 (p60) and 14281 (pl4). ORF 2 is thought to be expressed by suppression of the termination codon of ORF 1 to produce a M,. 189975 product (pl90). pl30 contains sequences characteristic of proteins with methyl transferase and NTP-binding properties and pl90 contains these and sequences characteristic of RNA-dependent RNA polymerases. The nucleotide sequence of IPCV RNA-1 is similar to that of peanut clump virus (PCV) and corresponding encoded polypeptides are 88% (pl30), 95% p60 and 75% (pl4) identical. The sequences of the translation products are also similar to those of soil-borne wheat mosaic virus and barley stripe mosaic virus. Oligonucleotide primers, designed on the basis of the sequences of RNA-1 of IPCV and PCV, were effective in reverse transcription-PCR amplification of these RNAs and that of IPCV isolates of the serologically distinct L and T serotypes.
 
Publisher Springer Verlag
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1574/1/ArchVirol_141_2301-2312_1996.pdf
Miller, J S and Wesley, S V and Naidu, R A and Reddy, D V R and Mayo, M A (1996) The nucleotide sequence of RNA-1 of Indian peanut clump furovius. Archives of Virology, 141 (1). pp. 2301-2312.