Record Details

The complete nucleotide sequence and genome organization of the M RNA segment of peanut bud necrosis tospovirus and comparison with other tospoviruses

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/1578/
http://vir.sgmjournals.org/content/77/9/2347.full.pdf
 
Title The complete nucleotide sequence and genome organization of the M RNA segment of peanut bud necrosis tospovirus and comparison with other tospoviruses
 
Creator Satyanarayana, T
Mitchell, S E
Reddy, D V R
Kresovich, S
Jarret, R
Naidu, R A
Gowda, S
Demski, J W
 
Subject Groundnut
 
Description The M RNA of peanut bud necrosis tospovirus is 4801 nucleotides in length. It comprised 2 ORFs in an ambisense organization and terminal inverted repeats. The 3 prime large ORF (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kDa which was identified as the glycoprotein precursor (GP) of the G1 and G2 glycoproteins. A comparison of the deduced amino acid sequence of GP revealed 37% identity and 58-59% similarity with that of tomato spotted wilt tospovirus (TSWV,serogroup I) and impatiens necrotic spot tospovirus (INSV, serogroup III) and 21-23% identity and 44-47% similarity with members of the genus Bunyavirus. The 5 prime small ORF (924 nucleotides in the virus-sense strand) encoded a 34.2 kDa protein which was identified as the non-structural (NSm) protein based on 41-43% identity and 60-63% similarity with TSWV and INSV. Defective RNA molecules derived from the genomic M RNA were detected during continuous passage of the virus by sap inoculations.
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/1578/1/Journal_of_General_Virology_77%289%29_2347-2352_1996.pdf
Satyanarayana, T and Mitchell, S E and Reddy, D V R and Kresovich, S and Jarret, R and Naidu, R A and Gowda, S and Demski, J W (1996) The complete nucleotide sequence and genome organization of the M RNA segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. Journal of General Virology, 77 (9). pp. 2347-2352.