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Detection of virulent and ESBL-resistant Salmonella species from cattle and associated farm environment

Indian Agricultural Research Journals

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Title Detection of virulent and ESBL-resistant Salmonella species from cattle and associated farm environment
 
Creator SUDRIK, SNEHAL
KOLHE, RAHUL
BHONG, CHANDRAKANT
SHENDE, TEJAS
JADHAV, SAMEER
MHASE, PRASHANT
WASKAR, VIKAS
HINGE, GANESH
GADHAVE, SNEHAL
 
Subject Antibiotic resistance, Cattle, ESBL, Salmonella, Virulence
 
Description The aim of this study was to explore the virulence characteristics and antibiotic resistance in Salmonella species isolated from cattle farms. During the study, 10 cattle farms were selected and from each farm, 35 samples consisting of faeces, milk, udder swabs, hand swabs, floor swabs, animal drinking water, pit water, composite fodder sample, and dairy utensil swabs were collected. Accordingly, 350 samples were collected from 10 farms and processed for the detection of Salmonella spp. Presumptive Salmonella isolates were confirmed by PCR and characterized for virulence and antibiotic resistance genes. From 350 samples, 19 (5.40%) Salmonellae were recovered, and out of 19 Salmonellae, 12 were ESBL producers. Similarly, out of 19 isolates, spvC was the predominant gene detected (42.10%), followed by stn (31.5%), and spvR (26.31%), whereas, invA, and iroB genes were detected in all 19 Salmonella isolates. Most of the Salmonella isolates were multi-drug resistant strains exhibiting resistance to nalidixic acid (94.73%), tetracycline (36.84%), and ceftazidime (42.10%). Out of 12 ESBL-positive Salmonellae, 9 (75%) isolates harboured ESBL genes, viz. blaSHV and blaTEM (25% each) and blaCTX-M and blaOXA (16.66% each). Six Salmonella isolates showed the presence of tetA gene. The current study indicated healthy cattle and the associated farm environments could be a source of virulent and drug-resistant Salmonellae. The current study also advocates phenotypic and genotypic antibiotic resistance monitoring at farm animal premises and agricultural ecosystems.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-10-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/141354
10.56093/ijans.v94i10.141354
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 10 (2024); 831–836
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/141354/56139
https://epubs.icar.org.in/index.php/IJAnS/article/view/141354/56140
 
Rights Copyright (c) 2024 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0