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Identification of a new MYBL2 isoform with distinct subcellular distribution

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Field Value
 
Title Identification of a new MYBL2 isoform with distinct subcellular distribution
 
Creator Zhang, Pei
Shen, Aiping
Mahmut, Maripat
Zhu, Na
Jiapaer, Zeyidan
 
Subject Human embryonic stem cells
Isoform
MYBL2
Neural stem cell
 
Description 620-626
Different splicing variants of genes may have different functions, and the identification of a new Splicing variant may broaden the role of the gene in many respects. MYBL2 plays a crucial role in the regulation of cell growth and differentiation, cell survival and cell cycle progression. In human embryonic stem cells (hESCs), there are usually two splicing forms of MYBL2 reported, MYBL2a and MYBL2b. Here, we successfully cloned the novel splicing variant of the MYBL2, which is named MYBL2c in vitro. MYBL2c lacks exon 3 and exon 4, as well as most of the regions encoding the DNA-binding domain. More importantly, MYBL2cis located in both the nucleus and cytoplasm, while MYBL2a is dominantly located in the nucleus. In addition, this splicing variant is not only expressed in undifferentiated human embryonic stem cells but also expressed in the neural stem cell, with a similar expression pattern to that of SOX2. Therefore, our study implies that the alternative splicing of MYBL2 is closely related to the maintenance of stem cell pluripotency.
 
Date 2024-10-04T06:25:38Z
2024-10-04T06:25:38Z
2024-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/64684
https://doi.org/10.56042/ijbb.v61i10.9564
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.61(10) [October 2024]