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Molecular marker (RAPD and RFLP) analysis of Mullet species

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Title Molecular marker (RAPD and RFLP) analysis of Mullet species
 
Creator Vidhya, I
Felix, N
Kathirvelpandian, A
Suresh, E
 
Subject 16s rRNA
Markers
Mullet
RAPD
RFLP
Taxonomy
 
Description 13-23
Mullet species belongs to the family, Mugilidae. Traditional taxonomic characters failed to differentiate mullet species
due to the possibility of cryptic species and morphological similarity among mullet species. This study aimed to differentiate
the selected mullet species using molecular markers. A total of 95 mullet samples (M. cephalus, P. macrolepis, and P. tade)
were collected from Tamil Nadu and Kerala. For this study, 5 RAPD primers (OPA1, OPA10, OPA11, OPA15, and OPA16)
were chosen from a set of 30 universal primers. The Nei’s unbiased genetic distance was calculated with the help of the
software “POPGEN version 1.32”. The genetic distance between P. tade and P. macrolepis was lower, while the distance
between M. cephalus and P. tade was greater, measuring 43.37 %. For the PCR-RFLP marker, the restriction enzyme was
chosen based on in-silico analysis. The Alu I enzyme displayed species-specific patterns in M. cephalus at 190 bp, 200 bp,
and 210 bp for the 16S rRNA gene.
 
Date 2024-10-09T09:39:22Z
2024-10-09T09:39:22Z
2024-01
 
Type Article
 
Identifier 2582-6727 (Online)
http://nopr.niscpr.res.in/handle/123456789/64709
https://doi.org/10.56042/ijms.v53i01.14108
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJMS Vol.53 (01) [January 2024]