Record Details

ISOLATION AND CHARACTERIZATION OF BIOFILM FORMING ESCHERICHIA COLI FROM CHICKEN MEAT SAMPLES

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title ISOLATION AND CHARACTERIZATION OF BIOFILM FORMING ESCHERICHIA COLI FROM CHICKEN MEAT SAMPLES
 
Creator I. Manikkavasagan
K. Vijayarani
B. Murugan
S. Meignanalakshmi
S. Eswari
 
Subject E. coli
Biofilm
Crystal violet assay
PCR
adhesion genes
 
Description The present study was aimed to investigate the presence of Escherichia coli (E.coli) in raw chicken meat samples collected from retail shops, as well as the biofilm-forming ability of field isolates, and to characterize different adhesion genes. Out of 20 chicken meat samples, 17 (85%) were positive for E. coli. Fifteen E. coli strains were characterized by PCR using the 16S rDNA primers and all the isolates were positive which confirmed that all the isolates were E.coli. Out of the 15 confirmed E.coli field isolates which were subjected to biofilm-forming assay, 46% of them were found to be strong biofilm producers. While all the isolates were screened for the presence of adhesion genes viz. luxS, csgA, fimH, fimA, and papC, the adhesion gene luxS was detected in all the strains (100%). The other adhesion genes csgA, fimH and fimA were detected in 93%, 93%, and 73% of the isolates, respectively. The E. coli field isolates were screened blaTEM gene was detected in only four strains, which was categorized under strong biofilm producers. This study demonstrated the presence of biofilm forming E. coli in the raw chicken meat samples as contaminants, causing spoilage and potentially posing risk to consumer’s health and safety.
 
Publisher Tamil Nadu Veterinary and Animal Sciences University
 
Date 2024-10-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJVASR/article/view/157991
10.56093/ijvasr.v53i4.157991
 
Source Indian Journal of Veterinary and Animal Sciences Research; Vol. 53 No. 4 (2024): IJVASR July - August 2024; 32-42
2347-2774
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJVASR/article/view/157991/56365
 
Rights Copyright (c) 2024 Indian Journal of Veterinary and Animal Sciences Research