Effect of Alpha-2 Agonists on the Locomotor Activity in Adult Zebrafish
Indian Agricultural Research Journals
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Title |
Effect of Alpha-2 Agonists on the Locomotor Activity in Adult Zebrafish
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Creator |
Saranya, R
Ramasamy, T Ramesh, S Srinivasan. M.R |
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Subject |
Alpha-2 agonists, Kinovea software, locomotor activity, zebra fish
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Description |
This study aimed to validate zebrafish as a suitable model for investigating the sedative impact of alpha-2 adrenergic receptor agonists. Zebrafish were exposed to xylazine (500 and 1000 µg/L), clonidine (50 and 500 µg/L), and dexmedetomidine (5 and 50 µg/L), and their locomotor activity was recorded using a Sony 13.6MP digital camera at 5-minute intervals up to 45 min post-exposure. Kinovea software was used to track and analyze the distance travelled and the swimming speed. Xylazine exhibited decreased locomotor activity at 500 µg/L but increased activity at 1000 µg/L, possibly indicating overlapping effects on α-1 receptor adrenergic receptors. Clonidine showed no significant reduction, possibly because of its lower affinity to the zebrafish α-2 receptor compared to the humanα-2 receptor, or could have lower absorption through the skin and gills due to the low logP value of clonidine. Dexmedetomidine significantly decreased the locomotor activity due to its 10 times higher α-2 adrenergic receptor selectivity as compared to xylazine and clonidine. These findings support the use of adult zebrafish as an alternative model to explore the effects of α-2 receptor agonists in drug discovery research.
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Publisher |
Indian Veterinary Association
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Date |
2024-10-22
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Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion |
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Format |
application/pdf
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Identifier |
https://epubs.icar.org.in/index.php/IVJ/article/view/158688
10.62757/IVA.2024.101.10.36-42 |
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Source |
The Indian Veterinary Journal; Vol. 101 No. 10 (2024): October, 2024; 36-42
0974-9365 0019-6479 |
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Language |
eng
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Relation |
https://epubs.icar.org.in/index.php/IVJ/article/view/158688/56626
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Rights |
Copyright (c) 2024 The Indian Veterinary Journal
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