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Effect of Alpha-2 Agonists on the Locomotor Activity in Adult Zebrafish

Indian Agricultural Research Journals

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Title Effect of Alpha-2 Agonists on the Locomotor Activity in Adult Zebrafish
 
Creator Saranya, R
Ramasamy, T
Ramesh, S
Srinivasan. M.R
 
Subject Alpha-2 agonists, Kinovea software, locomotor activity, zebra fish
 
Description This study aimed to validate zebrafish as a suitable model for investigating the sedative impact of alpha-2 adrenergic receptor agonists. Zebrafish were exposed to xylazine (500 and 1000 µg/L), clonidine (50 and 500 µg/L), and dexmedetomidine (5 and 50 µg/L), and their locomotor activity was recorded using a Sony 13.6MP digital camera at 5-minute intervals up to 45 min post-exposure. Kinovea software was used to track and analyze the distance travelled and the swimming speed. Xylazine exhibited decreased locomotor activity at 500 µg/L but increased activity at 1000 µg/L, possibly indicating overlapping effects on α-1 receptor adrenergic receptors. Clonidine showed no significant reduction, possibly because of its lower affinity to the zebrafish α-2 receptor compared to the humanα-2 receptor, or could have lower absorption through the skin and gills due to the low logP value of clonidine. Dexmedetomidine significantly decreased the locomotor activity due to its 10 times higher α-2 adrenergic receptor selectivity as compared to xylazine and clonidine. These findings support the use of adult zebrafish as an alternative model to explore the effects of α-2 receptor agonists in drug discovery research.
 
Publisher Indian Veterinary Association
 
Date 2024-10-22
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IVJ/article/view/158688
10.62757/IVA.2024.101.10.36-42
 
Source The Indian Veterinary Journal; Vol. 101 No. 10 (2024): October, 2024; 36-42
0974-9365
0019-6479
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IVJ/article/view/158688/56626
 
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