Record Details

Authentication of camel meat using species-specific PCR and PCR-RFLP

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Authentication of camel meat using species-specific PCR and PCR-RFLP
Not Available
 
Creator Vaithiyanathan, S., Vishnuraj, M.R., Narender Reddy, G. and Ch. Srinivas
 
Subject Camel Meat authentication Speciesspecific PCR-RFLP 12S rRNA CYTB gene Veterolegal
 
Description Not Available
In India and some of the African countries, one
of the unconventional meats receiving the latest attention in
meat adulteration is camel meat. So, the objective of this
study was to develop a species - specific PCR based on
mitochondrial cytochrome b (CYTB) gene and a PCRRFLP assay of mitochondrial 12S rRNA to identify camel
meat in suspected samples. Known sample of camel meat,
samples suspected to be from illegally slaughtered camel
and known samples of cattle, buffalo, sheep, goat, pork and
chicken were used in the study. DNA were extracted from
all samples following spin column method and PCR
amplification were carried out using both CYTB and 12S
rRNA gene primers. The CYTB gene amplification produced amplicon with a size of 435 bp without any nonspecific spurious amplification towards other species
studied. Further, the 12S rRNA PCR products were analysed both by sequencing and by RFLP using enzyme AluI.
On BLAST analysis the 448 bp sequence obtained from
suspected samples showed 99% sequence homology to
previously reported Camelus dromedaries (accession no:
AM 9369251.1). On AluI digestion of the 448 bp product
from both known and suspected camel samples, a specific
RFLP pattern with three distinct products of 90, 148 and
210 bp size were evident, which were significantly different from the pattern of cattle, sheep, goat, chicken and
buffalo. Further, after in - house validation, this cost
placed into practice for regular screening of vetero - legal
samples in the lab.
Not Available
 
Date 2024-11-01T08:39:23Z
2024-11-01T08:39:23Z
2020-10-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/84261
 
Language English
 
Relation Not Available;
 
Publisher Journal of Food Science and Technology