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Molecular Characterization and Antibiogram of Pseudomonas aeruginosa Isolated from Fish Sold in Markets of Tirupati, India: Molecular characterization and antibiogram of Pseudomonas aeruginosa

Indian Agricultural Research Journals

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Title Molecular Characterization and Antibiogram of Pseudomonas aeruginosa Isolated from Fish Sold in Markets of Tirupati, India: Molecular characterization and antibiogram of Pseudomonas aeruginosa
 
Creator Sowmya R
Jagadeesh Babu A.
Chinta, Siva Swetha
Sreedevi B.
Suresh Y.
Vinayaka Siddhartha P.
Madhava Rao T.
 
Subject Pseudomonas aeruginosa
molecular characterization
virulence genes
PCR
antimicrobial resistance
 
Description Pseudomonas aeruginosa is an emerging opportunistic pathogen and is one of the pathogens in the acronym ESKAPE representing a global threat to human health associated with antimicrobial resistance.  The present study aimed for molecular characterization, virulence gene profiling and antimicrobial resistance studies of P. aeruginosa isolated from 200 fish samples collected from different fish markets in Tirupati, Andhra Pradesh, India.  Of the 200 fish samples screened, 30.5% were confirmed as P. aeruginosa by PCR, targeting the 16S rRNA gene. Among the positive isolates, it was found that 70.4% and 75.4% of the isolates harbored toxA and exoS virulence markers, respectively.  All 61 P. aeruginosa isolates recovered were phenotypically characterized for antimicrobial resistance patterns by the disc diffusion method.  The study has found 100% resistance against erythromycin followed by 98.4% resistance to amoxicillin, 91.8% resistance against tetracycline, 88.5% resistance to vancomycin and cotrimoxazole, 45.9% resistance to ciprofloxacin, 44.2% resistance against cefepime and 9.9% resistance to gentamicin. The MAR indices were in the range from 0.5 to 1.0.  The prevalence of multiple antibiotic resistance of P. aeruginosa harboringvirulence genes in fish can pose a serious threat and hazard to public health through the consumption of raw or undercooked fish.
 
Publisher Society of Fisheries Technologists (India)
 
Date 2024-10-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/FT/article/view/152217
10.56093/ft.v61i4.152217
 
Source Fishery Technology; Vol. 61 No. 4 (2024): Fishery Technology
2582-2632
0015-3001
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/FT/article/view/152217/56913
 
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