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Studies on the nematicidal potentials of Pleurolobus gangeticus and Tragia involucrata

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Title Studies on the nematicidal potentials of Pleurolobus gangeticus and Tragia involucrata
 
Creator Menon, Madhuri
Mariya, Doono
Varghese, Leyon
 
Subject Anthelmintics
Ethnopharmacology
Haemonchus contortus
Molecular docking
Tubulin
 
Description 834-839
The study aimed at exploring and validating the nematicidal
potentials of Pleurolobus gangeticus and Tragia involucrata
against the gastrointestinal parasitic nematode of cattle called
Haemonchus contortus. Both plants are well known for their
ethno-pharmacological properties and have been used by various
traditional healers. Plant parts were extracted using ethyl acetate,
ethanol and water in an accelerated solvent extractor and the crude
extracts were evaluated for its nematicidal potentials against eggs
and L3 larvae of H. contortus using egg hatch inhibition assay and
larval paralysis assays respectively. The effects of the potent
extracts on mitotic machinery was evaluated in vitro by
performing an Allium cepa assay. Molecular docking analysis was
also performed to evaluate the binding affinity of
phytoconstituents to tubulin protein. The ethanol extracts of both
P. gangeticus (DME) and T. involucrata (TME) showed the
strongest inhibition of 90.83±0.98% and 90.5±1.64% respectively
on hatching of eggs at 10 mg/mL concentration. At this
concentration, larval paralysis assay also showed 65.5±2.25%
mortality for DME and 64.5±0.54% mortality for TME treated
nematodes. Ethyl acetate extracts of both plants showed relatively
less inhibition on egg hatch and larval paralysis and the aqueous
extracts were the least potent among the three. The Allium cepa
assay revealed an accumulation of cells in the metaphase when
treated with colchicine (32.3%) or extracts (DME-36.1%; TME-
35.5%) as compared to the vehicle controls (22.9%). In silico
analysis revealed that several components in DME and TME has
strong binding affinity with the colchicine binding site (CBS) of
tubulin protein complementing its observed anthelmintic
potential.
 
Date 2024-11-05T11:49:10Z
2024-11-05T11:49:10Z
2024-10
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64824
https://doi.org/10.56042/ijeb.v62i10.7630
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(10) [October 2024]