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Comparative analysis of tender and mature Hemigraphis colorata leaves on TGF-β activity in wound healing and molecular characterisation of plant extracts

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Title Comparative analysis of tender and mature Hemigraphis colorata leaves on TGF-β activity in wound healing and molecular characterisation of plant extracts
 
Creator Joy, Treesa
Krishnan, Akhila G
C, Mohankumar
Bhasker, Salini
 
Subject DDRT-PCR
Differentially expressed genes
Expressed sequence tags
HaCaT (Human keratinocytes) cells
RNA profiling
Sequence analysis
 
Description 815-823
With rising demand for effective and sustainable measures in
wound healing researchers are compelled to explore diverse
methods that combine folk medicine with existing wound care to
double the impact. Hemigraphis colorata (Blume) H G Hallier, an
exotic plant in the Acanthaceae family, is a perennial herb that has
potential therapeutic benefits. In the present investigation, a
comparative study was conducted between the effect of tender and
mature leaf extracts of H. colorata as well as the differentially
expressed genes of both leaves using four upstream primers and
one downstream (HT11C) primer. The rate of cell migration was
studied by creating a scratch wound in HaCaT monolayer culture.
The finding indicated that the application of tender leaf extract
resulted in a higher rate of wound closure, whereas the mature leaf
extract led to a two-fold increase in the secretion of TGF-β
protein, as measured by ELISA. This demonstrates the therapeutic
effectiveness of tender leaf extract in promoting wound healing
compared to mature leaf extract. Additionally, the results of
DDRT-PCR unveiled a diverse range of differentially expressed
genes, which were registered as molecular markers (ESTs) and
did not show homology with any known gene sequences in the
NCBI database.
 
Date 2024-11-05T11:52:32Z
2024-11-05T11:52:32Z
2024-10
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64826
https://doi.org/10.56042/ijeb.v62i10.1199
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(10) [October 2024]