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Taurine reduces cholesterol through CYP7A1 in a calcineurin-dependent manner

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Title Taurine reduces cholesterol through CYP7A1 in a calcineurin-dependent manner
 
Creator Guo, Junxia
Ou, Tong
Gao, Ya
Zhao, Yuxing
Zhang, Jing
Zhang, Yanzhen
Chen, Wen
 
Subject Calcineurin
Cholesterol
CYP7A1
MEK1/2
Taurine
 
Description 802-809
Taurine (2-aminoethanesulfonic acid) could reduce serum and liver cholesterol concentrations via regulating expression and
the activity of cholesterol 7α-hydroxylase (CYP7A1). To investigate the possible role of calcineurin in taurine upregulating
CYP7A1 expression in HepG2 cells under high cholesterol conditions. High cellular cholesterol conditions were achieved using
0.2 mM cholesterol in HepG2 cells. Calcineurin was decreased by FK506 and was depleted in CnAβ-/- cells. HepG2 cells were
cultured in a taurine-containing medium for 24 or 48 h. The levels of total intracellular cholesterol were determined by an
enzymatic method, and the expression levels of calcineurin, CYP7A1, and key transcriptional regulatory molecules were
detected by western blotting. High-cholesterol resulted in increased CYP7A1 and calcineurin expressions. Taurine exhibited
cholesterol-lowering effects regardless of low/high cholesterol conditions or calcineurin status. Taurine induced the expression
of CYP7A1, which was abolished by inhibiting or deleting calcineurin. Taurine suppressed MEK1/2, p-c-Jun, and SHP-1, key
molecules in one inhibitory pathway of CYP7A1 transcription. In contrast, suppression of MEK1/2 but not p-c-Jun or SHP-1
was reversed after completely knocking down calcineurin. Calcineurin is required for taurine’s upregulation of CYP7A1
expression through inhibiting MEK1/2, which was partly responsible for taurine’s cholesterol-lowering effect.
 
Date 2024-11-05T11:58:45Z
2024-11-05T11:58:45Z
2024-10
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64828
https://doi.org/10.56042/ijeb.v62i10.371
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(10) [October 2024]