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Fetal bovine serum vs platelet rich in growth factors: A comparative study on hematologic malignancies cell lines

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Title Fetal bovine serum vs platelet rich in growth factors: A comparative study on hematologic malignancies cell lines
 
Creator Salavatipour, Maryam Samareh
Kasgari, Fatemeh Hosseinpor
Zahedi, Amir Mohammad
Valandani, Hajar Mardani
Khalilabadi, Roohollah Mirzaee
 
Subject Cell culture techniques
FBS alternatives
Tumour cell line
Xeno-free serum
 
Description 788-794
Fetal bovine serum (FBS) is a popular cell culture supplement with growth, proliferation, and adhesion factors, but its
high costs, safety concerns, potential xenogeneic agent transmission, and accessibility issues discourage its use. Alternative
options have been proposed, each with its own advantages and disadvantages. However, caution is advised when choosing a
substitute, as alterations to the fundamental characteristics of cultured cells may introduce biases and impact clinical
applications. Herein, the authors assess the function of 10% PRGF compared to 10% FBS and 5% FBS+5% PRGF by
examining morphology, viability, and apoptosis rates in the NALM6, NB4, and U266 cell lines. Despite there being no
observable variance in cell morphology across the three cell lines, NALM6 and NB4 cells exhibited greater viability after 24
and 48 h of incubation in the presence of 10% PRGF. Meanwhile, the proliferation rate of NALM6 and NB4 cells in the
combination group and U266 cells across all groups remained comparable to that of the 10% FBS group. Furthermore, no
significant incidence of apoptosis was detected among the cultured cells at the desired additive concentrations. Our study
showed that PRGF could be recognised as an optimal, accessible, safe, and affordable supplement for FBS.
 
Date 2024-11-05T12:02:49Z
2024-11-05T12:02:49Z
2024-10
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/64830
https://doi.org/10.56042/ijeb.v62i10.3983
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJEB Vol.62(10) [October 2024]