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ENHANCED LIPASE PRODUCTION FROM CELOSIA ARGENTEA SEED OIL USING MUTANT BACILLUS PUMILUS UVM6

Indian Agricultural Research Journals

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Title ENHANCED LIPASE PRODUCTION FROM CELOSIA ARGENTEA SEED OIL USING MUTANT BACILLUS PUMILUS UVM6
 
Creator P. SATHIYA
K. DHANDAYUTHAPANI
 
Subject Bacillus pumilus
Celosia argentea seed oil
lipase
mutation
submerged fermentation
 
Description A mutant strain was developed by random mutation using UV-C radiation from wild parent  strain Bacillus pumilus KMAS7. Following mutagenesis, eleven bacterial isolates were used for maximum lipase production and found that the strain UVM6 produced high lipase of 36.46±0.05 UmL-1 from C. argentea seed oil. It was 1.38 times more abundant than the wild parent strain. Robust mutant strain UVM6 was selected and designated as B. pumilus UVM6. Then this mutant strain was cultivated in SmF for production of lipase using C. argentea seed oil under different nutritional sources. An overall 2.95-fold enhanced extracellular lipase production (62.42±0.03 UmL-1) was obtained from C. argentea seed oil by SmF using 4% v/v C. argentea seed oil, 0.5% w/v glucose, 1% w/v ammonium sulphate and 10 μML-1 Zn as inducer, carbon, nitrogen and trace element sources respectively. Seed oil of C. argentea has been used in traditional folk medicine, but this is the first time we’re reporting lipase production from C. argentea by the newly isolated mutant B. pumilus UVM6.
 
Publisher Acharya N. G. Ranga Agricultural University, Guntur
 
Date 2024-06-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/TJRA/article/view/160067
10.58537/
 
Source The Journal of Research ANGRAU; Vol. 52 No. 2 (2024): The Journal of Research ANGRAU, Vol. LII No. (2), pp.1-160, April - June, 2024; 32-41
0970-0226
10.58537/jorangrau.2024.52.2
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/TJRA/article/view/160067/57251
 
Rights https://creativecommons.org/licenses/by-nc-sa/4.0