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A simple method for long-term preservation of cultures of Colletotrichum graminicola and C. gloeosporioides [Glomeralla cingulata] causing anthracnose in sorghum

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Title A simple method for long-term preservation of cultures of Colletotrichum graminicola and C. gloeosporioides [Glomeralla cingulata] causing anthracnose in sorghum
 
Creator Mathur, K
Thakur, R P
Rao, V P
 
Subject Sorghum
 
Description Long-term preservation and maintenance of pathogen isolates is essential for studies on pathogenic variability and virulence monitoring. Once the pathogen isolates are appropriately characterized for their morphologic, pathogenic, and genetic diversity they become reference isolates for future studies. Frequent subculturing of necrotrophic pathogens is not only laborious, but it can also induce spontaneous natural mutations in actively growing cultures. There are numerous reports of reduced viability and pathogenicity of isolates due to repeated subculturing on synthetic media. In our studies on pathogenic variability in Colletotrichum graminicola (Ces.) Wils. and C. gloeosporioides (Penz.) Sacc., the causal agents of anthracnose of sorghum (Mathur et al. 1998), we faced the problem of adequately maintaining a large number of isolates (>200) by repeated subculturing on oatmeal agar (OMA) at monthly intervals.
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1781/1/ISMN40_43-44_1999.pdf
Mathur, K and Thakur, R P and Rao, V P (1999) A simple method for long-term preservation of cultures of Colletotrichum graminicola and C. gloeosporioides [Glomeralla cingulata] causing anthracnose in sorghum. International Sorghum and Millets Newsletter, 40. pp. 43-44.