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Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus

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http://vir.sgmjournals.org/content/81/1/267.full.pdf+html
 
Title Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus
 
Creator Bragard, C
Duncan, G H
Wesley, S V
Naidu, R A
Mayo, M A
 
Subject Groundnut
 
Description cDNA copies of the coat protein (CP) gene of Indian peanut clump virus (IPCV)-H were introduced into cells of Nicotiana benthamiana or Escherichia coli by transformation with vectors based on pROKII or pET respectively. In both plant and bacterial cells, IPCV CP was expressed and assembled to form virus-like particles (VLP). In plant extracts, the smallest preponderant particle length was c. 50 nm. Other abundant lengths were c. 85 and c. 120 nm. The commonest VLP length in bacterial extracts was c. 30 nm. Many of the longer VLP appeared to comprise aggregates of shorter particles. The lengths of the supposed 'monomer' VLP corresponded approximately to those expected for encapsidated CP gene transcript RNA. Immunocapture RT-PCR, using primers designed to amplify the CP gene, confirmed that the VLP contained RNA encoding IPCV-H CP. The results show that encapsidation does not require the presence of the 5′-terminal untranslated sequence of the virus RNA and suggest that if there is an 'origin of assembly' motif or sequence, it lies within the CP gene. When transgenic plants expressing IPCV-H CP were inoculated with IPCV-L, a strain that is serologically distinct from IPCV-H, the virus particles that accumulated contained both types of CP.
 
Publisher Society for General Microbiology
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1891/1/JGenVirol81%281%29267-272.pdf
Bragard, C and Duncan, G H and Wesley, S V and Naidu, R A and Mayo, M A (2000) Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus. Journal of General Virology, 81 (1). pp. 267-272. ISSN 0022-1317