ICAR Research Data Repository for Knowledge Management
A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/1921/
http://dx.doi.org/10.1007/BF02825068 |
|
| Title |
A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications
|
|
| Creator |
Sharma, K K
Lavanya, M Anjaiah, V |
|
| Subject |
Groundnut
|
|
| Description |
Numerous methods are available for isolation of plant genomic DNA, but in practice these procedures are empirical due to variability in plant tissue composition. Consistent isolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematic due to the presence of phenolic compounds and polysaccharides. Inconsistencies in extraction results can be attributed to the age and growth stage of the plant material analyzed. Mature leaves have higher quantities of polyphenols, tannins, and polysaccharides that can contaminate DNA during isolation. We show that four published protocols could not be used to isolate peanut DNA of sufficient quality for PCR amplification or Southern hybridization. We have devised a new protocol that uses DEAE-cellulose purification to isolate peanut DNA useful for downstream applications. |
|
| Publisher |
Springer
|
|
| Date |
2000
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Rights |
—
|
|
| Identifier |
http://oar.icrisat.org/1921/1/PlMolBioRep18_4_393a-309h_2000.pdf
Sharma, K K and Lavanya, M and Anjaiah, V (2000) A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications. Plant Molecular Biology Reporter, 18 (4). 393a-393h. ISSN 1572-9818 |
|