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An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation

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Relation http://oar.icrisat.org/1922/
http://dx.doi.org/10.1016/S0168-9452(00)00294-6
 
Title An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation
 
Creator Sharma, K K
Anjaiah, V
 
Subject Groundnut
 
Description Cotyledon explants from mature peanut seeds (Arachis hypogaea L.) were optimized to obtain adventitious shoot buds with
high frequencies (\90%). Efficient transformation of these cotyledons by using Agrobacterium tumefaciens strain C58 carrying
neomycin phosphotransferase II (nptII) and ß-glucuronidase (GUS; uidA), or coat protein gene of the Indian peanut clump virus
(IPCVcp) and nptII on binary vectors (pBI121; pROKII:IPCVcp) led to the production of a large percentage (55%) of transgenic
plants. Transformed individuals were obtained through selection on medium containing 125 mg l�1 kanamycin. A large number
of independently transformed plants (over 75) were successfully transplanted to the glasshouse. Integration of the transgenes and
stable genetic transformants in the progeny were assessed by PCR amplification of 700-bp fragment of nptII and 585-bp of
IPCVcp genes, and Southern blot hybridizations in the T1 generation of transgenic plants. Analysis of 35 transgenic plants of T1
generation from the progeny of a single transformation event suggested the segregation of a single copy insert in a 3:1 Mendelian
ratio. On an average, 120–150 days were required between the initiation of explant transformation and transfer of rooted plants
to the greenhouse. The cotyledon regeneration system proved to be an excellent vehicle for the production of a large number of
independently transformed peanut plants. Shoot formation was rapid and prolific, and a large proportion of these shoots
developed into fertile plants. The method reported here provides new opportunities for the crop improvement of peanut via
genetic transformation. © 2000 Elsevier Science Ireland Ltd. All rights reserved.
 
Publisher Elsevier
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/1922/1/PlSci159_1_7-19_2000.pdf
Sharma, K K and Anjaiah, V (2000) An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation. Plant Science, 159 (1). pp. 7-19. ISSN 0168-9452