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Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay

OAR@ICRISAT

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Relation http://oar.icrisat.org/1996/
http://www.nrcresearchpress.com/doi/pdf/10.1139/g00-034
 
Title Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay
 
Creator Subramanian, V
Gurtu, S
Rao, R C N
Nigam, S N
 
Subject Groundnut
 
Description Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement programme. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing a RAPD assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yielded polymorphic amplification products. The total number of bands from the 7 primers was 408, of which 27 were polymorphic. Detection of polymorphism in cultivated groundnut opens up the possibility of development of its molecular map by judicious selection of genotypes that show DNA polymorphism. This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits
 
Publisher NRC Press
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/1996/1/Genome_43_4_656-660_2000.pdf
Subramanian, V and Gurtu, S and Rao, R C N and Nigam, S N (2000) Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay. Genome, 43 (4). pp. 656-660.