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Detached leaf assay to evaluate transgenic pigeonpea plants for resistance to Helicoverpa armigera

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http://whalonlab.msu.edu/Newsletter/index.html
 
Title Detached leaf assay to evaluate transgenic pigeonpea plants for resistance to Helicoverpa armigera
 
Creator Gopal Swamy, S V S
Sharma, H C
Sharma, K K
Subbaratnam, G V
Kumar, C S
 
Subject Pigeonpea
 
Description Introduction of transgenic insect-resistant pigeonpea (Cajanus cajan (L.) Millsp.), is expected to be useful in minimizing Helicoverpa armigera (Hubner) damage, which is the major constraint for its production. In this context, it is important to develop techniques to evaluate effectiveness of transgenic plants for resistance to H. armigera. Therefore, we evaluated the usefulness of detached leaf assay to assess the efficacy of transgenic pigeonpea (var; ICPL 88039 and ICPL 87) plants carrying Btcry1Ab and SBTI genes for resistance to H. armigera. The levels of Cry1Ab or SBTI proteins in the transgenic pigeonpea plants were not sufficient to cause significant deterrent effects on leaf feeding, larval survival, and larval weight of H. armigera. However, detached leaf assay was found to be quite useful for evaluation of transgenic pigeonpea plants for resistance to H. armigera.
 
Publisher Spring
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/2083/1/Detached_leaf_assay_to_evaluate_transgenic_pigeonpea_plants_for_resistance_to_Helicoverpa_armigera.pdf
Gopal Swamy, S V S and Sharma, H C and Sharma, K K and Subbaratnam, G V and Kumar, C S (2007) Detached leaf assay to evaluate transgenic pigeonpea plants for resistance to Helicoverpa armigera. Resistant Pest Management Newsletter, 16 (2). pp. 24-27. ISSN 1061-7795