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Phenotypic and genetic diversity in the foxtail millet (setaria italica (l.) p. beauv.) core collection)

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Title Phenotypic and genetic diversity in the foxtail millet
(setaria italica (l.) p. beauv.) core collection)
 
Creator Vetriventhan, M
 
Subject Millets
 
Description Foxtail millet core collection consisting of 155 accessions was evaluated at three
environments for 12 qualitative and 13 quantitative traits to study the phenotypic diversity and to
identify trait specific accessions. Foxtail millet core collection was also molecularly profiled
using 84 SSR markers to study molecular genetic diversity, population structure and to identify
SSR markers associated with the agronomic traits.
In REML analysis variance due to genotypes (σ2
g) and genotype × environment (σ2
ge)
were significant for all the 13 quantitative traits. On the basis of phenotypic dissimilarity
between pair of accessions, ten pairs of most diverse accessions were identified for breeding
program for the developing high yielding cultivars with a broad genetic base and for the
development of mapping populations. On the basis of pooled BLUPs (Best Linear Unbiased
Predictors) of three environments, we have identified trait specific accessions for economically
important traits such as yield and its traits contributing to yield (15 accessions for each trait).
These accessions could be used in recombination breeding to develop cultivars with desirable
combination of traits.
The SSR markers detected a total of 1,356 alleles with an average of 16.14 alleles per
locus. Of these, 368 were rare alleles; 906 common alleles; and 82 the most frequent alleles.
Sixty one unique alleles which were specific to a particular accession and useful for germplasm
identification were also detected. The genetic diversity of foxtail millet in this study was
correlated well with racial classification and the race indica showed greater genetic distance
from the maxima and moharia. Ten pairs of genetically most diverse accessions were identified.
Large molecular variation observed in core collection could be utilized effectively for selection
of diverse parents for breeding cultivars and development of mapping populations. Mantel test
showed significant correlation between phenotypic and molecular dissimilarity matrix.
The STRUCTURE analysis provided the evidence for the presence of four
subpopulations. The mixed linear model (MLM) was used and the number of significant marker
trait association was 130 in E1, 69 in E2 and 106 in E3 at P≤0.05, whereas only 49 in E1, 23 in
E2 and 61 in E3 were found to be highly significant MTAs at P≤0.01. In pooled BLUPs of three
environments, a total of 108 MTAs were detected at P≤0.05. Of these 18 SSR markers showed
37 significant associations at P≤0.01 with yield and yield contributing traits. Fifteen MTAs, that
occurred in all three environments and overall in pooled data were identified as stable. Our
research provided a first report of association study for yield and yield contributing traits in
foxtail millet using SSR markers. The results from this research also demonstrated the use of
core collection as association mapping panel to disclose marker-trait associations in foxtail millet
for yield traits that could lead to effective utilization of ex-situ conserved genetic resources.
 
Date 2011
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/2277/1/Mr_Vetriventhan.pdf
Vetriventhan, M (2011) Phenotypic and genetic diversity in the foxtail millet (setaria italica (l.) p. beauv.) core collection). PhD thesis, Tamil Nadu Agricultural University.