Record Details

Biotechnology and chickpea breeding

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/2451/
 
Title Biotechnology and chickpea breeding
 
Creator Rheenen, H A van
 
Subject Chickpea
 
Description Isoenzyme, RFLP and randomly amplified polymorphic DNA (RAPD) analyses were carried out on 2 sets of chickpea (Cicer arietinum) varieties. Isoenzyme analysis of a number of enzymes in both sets detected only one polymorphism, for alanyl naphthlyamide aminopeptidase, RFLP analysis failed to show polymorphism, while RAPD analysis successfully detected varietal differences and gave sharp banding patterns; DNA of 3 of the 8 varieties in one set showed polymorphism when hybridized with 14 different primers containing 10 bases each, while all 4 primers used for the second set of varieties showed polymorphism. Thus, these primers with short sequences were able to detect genomic variations at relatively high frequency.
 
Date 1992
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights
 
Identifier http://oar.icrisat.org/2451/1/ICN26%252814-17%25291992.pdf
Rheenen, H A van (1992) Biotechnology and chickpea breeding. International Chickpea Newsletter, 26. pp. 14-17.