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Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum

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Relation http://oar.icrisat.org/2574/
 
Title Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum
 
Creator Girijashankar, V
Sharma, K K
Balakrishna, P
Seetharama, N
 
Subject Sorghum
 
Description Sorghum (Sorghum bicolor) is an important staple food
crop, especially of the poor in Africa, Asia and Central
America and needs to be improved through genetic
engineering. Generation of transgenic plants depends
mainly on the standardization of an efficient tissue culture
and regeneration protocol. Sorghum tissue culture and
regeneration protocols mostly follow either organogenesis
(Maheswari et al. 2006) or direct somatic embryogenesis
pathway (Harshavardhan et al. 2002). Following the
protocol of Girijashankar et al. (2005), we attempted to
produce transgenic sorghum with synthetic Bt gene
constructs (ubicry1Ab and ubicry1Ac, separately).
However, molecular analysis of the T0 and their progeny
plants revealed the loss of germline transmission of the
respective transgenes under study. Recent studies by
Wang et al. (2005) with Agrobacterium-mediated
transformation of maize (Zea mays) and regeneration
through embryogenic callus, reported the absence of
transgenes in the progeny plants. We report here the
occurrence of different types of calli, few of which are
not expected to occur during the direct somatic
embryogenesis pathway of sorghum regeneration
protocol followed to obtain the above transgenic plants.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/2574/1/Direct_somatic_embryogenesis.pdf
Girijashankar, V and Sharma, K K and Balakrishna, P and Seetharama, N (2007) Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum. Journal of SAT Agricultural Research, 3 (1). pp. 1-3.