ICAR Research Data Repository for Knowledge Management
Complementary DNA probe for the detection of isolates of Indian peanut clump virus
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/2666/
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| Title |
Complementary DNA probe for the detection of isolates of Indian peanut clump virus
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| Creator |
Balan, K V P
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| Subject |
Groundnut
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| Description |
The main aim of the present study was to use the clones of PCV genome and select a clone which would hybridize with RNA of several PCV isolates which could be used in the preparation of a C-DNA probe. For this purpose Northern Dot Blot hybridization technique was used which was probed with nick translated 32 p. labeled C-DNA clones of H-IPCV. Among the H-IPCV clines analyzed the 2 kbp and 3.5-4 kbp clines were employed in this study to probe the IPCV isolates. Autoradiographic signals showed greater hybridization could be achieved with 3.5-4 kbp clone than 2 kbp clone using the pure virus and nucleic acid of H-IPCV and with RNA of partially purified preparations of L-IPCV and total nucleic acids of B-IPCV isolates
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| Date |
1989
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| Type |
Thesis
NonPeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/2666/1/59553.pdf
Balan, K V P (1989) Complementary DNA probe for the detection of isolates of Indian peanut clump virus. Masters thesis, Indian Institute of Technology Kharagpur, West Bengal. |
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