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Purification and some serological relationships of tomato spotted wilt virus isolates occurring on peanut (Arachis hypogaea) in the USA

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Relation http://oar.icrisat.org/2764/
 
Title Purification and some serological relationships of tomato spotted wilt virus isolates occurring on peanut (Arachis hypogaea) in the USA
 
Creator Sreenivasulu, P
Demski, J W
Reddy, D V R
Naidu, R A
Ratna, A S
 
Subject Plant Pathology
 
Description A procedure for the purification of TSWV-Tx, a tomato spotted wilt virus isolate infecting groundnuts in Texas, is described. A rabbit antiserum was produced. Several TSWV isolates occurring on groundnuts in the USA reacted to varying extents in ELISA with antisera to TSWV-Tx and to Greek TSWV isolates, but failed to react with antiserum to an isolate of TSWV from India. In reciprocal tests, antigens of the Indian TSWV failed to react with antisera to Tx and to the Greek isolates. Purified TSWV-Tx contained 4 polypeptide species of 78 000, 54 000 and 27 000 Da. In electro-blot immunoassays, all 4 polypeptides reacted with the homologous antiserum and with antisera prepared against a Greek, a Dutch and an Australian isolate. None of the polypeptides reacted with the antiserum to the Indian isolate.
 
Date 1991
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/2764/1/Purification%2520and%2520some%2520serological%2520relationships.pdf
Sreenivasulu, P and Demski, J W and Reddy, D V R and Naidu, R A and Ratna, A S (1991) Purification and some serological relationships of tomato spotted wilt virus isolates occurring on peanut (Arachis hypogaea) in the USA. Plant Pathology, 40 (4). pp. 503-507.