ICAR Research Data Repository for Knowledge Management
An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea
OAR@ICRISAT
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| Relation |
http://oar.icrisat.org/2855/
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| Title |
An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea
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| Creator |
Shivanna, K R
Saxena, N P Seetharama, N |
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| Subject |
Chickpea
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| Description |
Using pollen from a cold tolerant accession ICC8923, best results for pollen germination and pollen tube growth were obtained in medium containing 2.5% sucrose + 10% PEG + 100 mg boric acid + 300 mg calcium nitrate + 200 mg magnesium sulfate + 100 mg potassium nitrate/litre. The results of pollen germination tests using this medium (PEG medium) and Brewbaker & Kwack (BK) medium are reported. In the BK medium, pollen germination was lower and pollen tube growth was largely confined to the first 60 min with tube abnormalities such as swelling and bursting occurring in 3-h-old cultures. In PEG medium pollen germination was consistently high (
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| Publisher |
International Crops Research Institute for the Semi-Arid Tropics
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| Date |
1997
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/2855/1/ICPL4-28-29%25281997%2529.pdf
Shivanna, K R and Saxena, N P and Seetharama, N (1997) An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea. International Chickpea Newsletter, 4. pp. 28-29. ISSN 0257-2508 |
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