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RFLP Analysis of Cytoplasmic-Genic Male-Sterile Lines of Pigeonpea Developed by Interspecific Crosses

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Relation http://oar.icrisat.org/2942/
 
Title RFLP Analysis of Cytoplasmic-Genic Male-Sterile Lines of Pigeonpea Developed by Interspecific Crosses
 
Creator Sivaramakrishnan, S
Seetha, K
Rao, A N
Singh, L
 
Subject Pigeonpea
 
Description To study the differences between genetically (GMS) and cytoplasmically male sterile (CMS) lines in pigeon pea (Cajanus cajan), RFLP analysis of total genomic DNA from 3 putative CMS progenies derived from crosses between the wild species C. sericeus and cultivated pigeon pea, and 7 C. cajan accessions (including 2 GMS lines) was performed. After the initial cross, C. sericeus EC121208 × C. cajan ICPX880227, transfer of the nuclear genome of pigeonpea into C. sericeus cytoplasm was achieved by 5-6 subsequent genomic transfer stages using different male pigeon pea parents. Hybridization patterns clearly distinguished between GMS lines ICPL87 and MS Prabhat, CMS lines produced by multiple gene transfer and pigeon pea lines, although there were also differences in the restriction fragment patterns among the various pigeon pea cultivars, and between MS Prabhat and ICPL87. Results suggest that crosses between C. sericeus and cultivated pigeonpea produced male sterile lines having mitochondria of the latter.
 
Publisher International Crops Research Institute for the Semi-Arid Tropics
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/2942/1/ICPN3%2528103-106%25291996.pdf
Sivaramakrishnan, S and Seetha, K and Rao, A N and Singh, L (1996) RFLP Analysis of Cytoplasmic-Genic Male-Sterile Lines of Pigeonpea Developed by Interspecific Crosses. International Chickpea Newsletter, 3. pp. 103-106. ISSN 0257-2508