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Random Amplified Microsatellite Polymorphism (RAMPO) Analysis of Chickpea Accessions

OAR@ICRISAT

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Relation http://oar.icrisat.org/2947/
 
Title Random Amplified Microsatellite Polymorphism (RAMPO) Analysis of Chickpea Accessions
 
Creator Banerjee, H
Leela, A
Seetharama, N
 
Subject Chickpea
 
Description DNA was isolated from 4 cultivated Cicer arietinum accessions and one wild C. echinospermum accession using the CTAB method. RAPD analysis with 12 primers revealed 424 amplified DNA bands. The wild species produced a maximum of 34 polymorphic bands, while all cultivars except ICCV92504 produced 2 or 3 polymorphic bands. RAMPO analysis of these accessions using microsatellite probes revealed amplified bands not visible on ethidium [homidium] bromide-stained gel. Combination of RAPD analysis and probing with microsatellite repeats (TG)10, (GATA)4 and (CAC)5 revealed polymorphic and genotype-specific unique DNA banding patterns. Thus, all 5 chickpea accessions could be distinguished using RAMPO.
 
Publisher International Crops Research Institute for the Semi-Arid Tropics
 
Date 1998
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/2947/1/ICPN5%252816-18%25291995.pdf
Banerjee, H and Leela, A and Seetharama, N (1998) Random Amplified Microsatellite Polymorphism (RAMPO) Analysis of Chickpea Accessions. International Chickpea Newsletter, 5. pp. 16-18. ISSN 0257-2508