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Development and Utilization of Genetic Diversity Based Ethiopian Chickpea (Cicer arietinum L.) Germplasm Core Collection for Association Mapping

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Relation http://oar.icrisat.org/2964/
 
Title Development and Utilization of Genetic Diversity Based Ethiopian Chickpea (Cicer arietinum L.) Germplasm Core Collection for Association Mapping
 
Creator Kibret, K T
 
Subject Chickpea
 
Description Chickpea (Cicer arietinum L) is one of the most important cool season grain legume crops grown in semi-
arid tropics and Mediterranean regions. Terminal drought stress is one of the limiting factors for chickpea
production. Utilizing of germplasm collections are the main gateway to improve the stagnant production
of chickpea in semi arid tropics.
Hence, the objectives of this study were to i) Preliminary phenotyping and genotyping of germplasms
collections for diversity assessment; ii) Development of chickpea core collection based on diversity
analysis; iii) Identification of desirable accessions for drought tolerance from core set by proper
phenotyping; iv) Large scale genotyping of the core collections by SNP markers; v) Large scale
genotyping of the core collections by SNP markers; vi) Identification and establishing marker trait
associations using appropriate association genetic approaches; vii) Quantification of population structure
and relationship of Ethiopian chickpea collection.
The phenotypic evaluation in contrasting environment and SNP marker data analysis revealed that there is
significant phenotypic and genotypic variability in Ethiopian chickpea germplasm for drought tolerance
and other agronomic traits. The population structure and relationship analysis also revealed strong
subpopulation fixation and differentiation which was significantly different from the original population.
High allelic and gene diversity were observed in the entire collection with common and rare alleles. Trait
marker association analysis showed markers which are strongly associated with maturity related traits and
high linkage disequilibrium observed for the polymorphic markers.
Core collection for Ethiopian chickpea germplasm were developed and validated for different validation
parameters such as percent mean difference (MD %), percent variance difference (VD %), analysis of
variance, coincidence rate of range (CR %), variable rate of coefficient of variance (VR %) and genetic
diversity index. The result of validation showed better correspondence between the core set and the entire
set which had avoided germplasm duplication and representing the whole collection economically in time
and money with few numbers of accessions. Drought tolerant accessions were also identified in the
preliminary field screening which needs further confirmation.
 
Publisher International Crops Research Institute for the Semi-Arid Tropics
 
Date 2011
 
Type Thesis
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/2964/1/KebedeTeshomeKibret.pdf
Kibret, K T (2011) Development and Utilization of Genetic Diversity Based Ethiopian Chickpea (Cicer arietinum L.) Germplasm Core Collection for Association Mapping. PhD thesis, International Crops Research Institute for the Semi-Arid Tropics.